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Image Search Results
Journal: bioRxiv
Article Title: Spatial and temporal control of expression with light-gated LOV-LexA
doi: 10.1101/2021.10.19.465021
Figure Lengend Snippet: A . Schematics showing the timeline of the experiment, light regime as well as the construct selected for LOV-LexA. B-G . Fat bodies of second to third instar larvae with LOV-LexA expression controlled by Cg-GAL4 for larvae kept in the dark (B-D) and exposed to three 30s pulses of blue LED light at 1 Hz (E-G). Exposure to blue light appears to alter LOV-LexA cellular distribution (C, F) and leads to expression of LexAop-CsChrimson:Venus in fat body cells as detected with anti-GFP antibody (D, G). G . Exposure to blue light leads to an increase in the amount of green signal, derived from CsChrimson:Venus compared to the LOV-LexA signal, measured by the ratio of anti-GFP signal/anti-RFP signal.
Article Snippet: Cells were fixed with 4% paraformaldehyde overnight at 4°C, between 8 to 10 hours after addition of copper sulfate, and processed for immunostaining with standard protocols [for eg., 97], with
Techniques: Construct, Expressing, Derivative Assay
Journal: Immunity
Article Title: Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche
doi: 10.1016/j.immuni.2019.08.017
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Recombinant, Blocking Assay, Irradiation, Enzyme-linked Immunosorbent Assay, cDNA Synthesis, Microarray, Software, Microscopy